D Behrens
Loss of tumourigenicity of stably ERβ-transfected MCF-7 breast cancer cells
Behrens, D; Gill, J.H; Fichtner, I
Authors
J.H Gill
I Fichtner
Abstract
Proliferation of breast cancer cells is mediated by estrogen receptors (ER)-ERalpha and ERbeta. At present, contradictory observations complicate the understanding of involvement of ERbeta in breast cancer and functional definition of ERbeta as a prognostic marker. A stable expression of full length ERbeta was established in the ERalpha-positive MCF-7 breast carcinoma cell line to evaluate the role for ERbeta in maintenance of cell viability and estrogenic response, as well as proliferation, morphology and cell cycle progression. In order to verify in vivo tumourigenicity of ERbeta transfectants were transplanted into nude mice. Transfection of ERbeta in MCF-7 resulted in a marginal increase of gelsolin protein expression. Constitutive expression of ERbeta resulted in a significant 30% inhibition of cellular growth compared with transfection of the mock vector alone (p=0.043). This reduction in growth was associated a retardation of transition into S-phase of the cell cycle. The in vitro response to 17beta-estradiol was reversed in cells over-expressing ERbeta (p=0.016). However, no difference in response to the antiestrogens tamoxifen and ICI 182,780 was observed in the presence of ERbeta. Importantly, over-expression of ERbeta prevented establishment and growth of tumours as subcutaneous xenografts in immunodeficient mice in vivo. These observations support the notion that ERbeta is a tumour suppressor and is exploitable in terms of cancer prevention, improving therapeutic response or predicting disease progression.
Citation
Behrens, D., Gill, J., & Fichtner, I. (2007). Loss of tumourigenicity of stably ERβ-transfected MCF-7 breast cancer cells. Molecular and Cellular Endocrinology, 274(1-2), 19-29. https://doi.org/10.1016/j.mce.2007.05.012
Journal Article Type | Article |
---|---|
Publication Date | Sep 1, 2007 |
Deposit Date | Jan 7, 2013 |
Publicly Available Date | Feb 12, 2013 |
Journal | Molecular and Cellular Endocrinology |
Print ISSN | 0303-7207 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 274 |
Issue | 1-2 |
Pages | 19-29 |
DOI | https://doi.org/10.1016/j.mce.2007.05.012 |
Keywords | Breast cancer, Cell cycle, Estrogen receptors, Xenograft. |
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Copyright Statement
NOTICE: this is the author’s version of a work that was accepted for publication in Molecular and cellular endocrinology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular and cellular endocrinology, 274, 1-2, 2007, 10.1016/j.mce.2007.05.012
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