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5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

Skipsey, M.; Hack, G.; Hooper, T.A.; Shankey, M.C.; Conway, L.P.; Schröder, M.; Hodgson, D.R.W.

5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs Thumbnail


Authors

M. Skipsey

G. Hack

T.A. Hooper

M.C. Shankey

L.P. Conway



Abstract

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

Citation

Skipsey, M., Hack, G., Hooper, T., Shankey, M., Conway, L., Schröder, M., & Hodgson, D. (2013). 5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs. Nucleosides, Nucleotides and Nucleic Acids, 32(12), 670-681. https://doi.org/10.1080/15257770.2013.851393

Journal Article Type Article
Publication Date Dec 11, 2013
Deposit Date Sep 30, 2013
Publicly Available Date Mar 29, 2024
Journal Nucleosides, Nucleotides and Nucleic Acids
Print ISSN 1525-7770
Electronic ISSN 1532-2335
Publisher Taylor and Francis Group
Peer Reviewed Peer Reviewed
Volume 32
Issue 12
Pages 670-681
DOI https://doi.org/10.1080/15257770.2013.851393
Keywords T7 RNA polymerase initiator, Fluorophore-labeled RNA.

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Copyright Statement
© Skipsey et al. Published with License by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.





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