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The Arabidopsis Mediator complex subunits MED16, MED14 and MED2 regulate Mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes

Hemsley, P.A.; Hurst, C.H.; Kaliyadasa, E.; Lamb, R.; Knight, M.R.; De Cothi, E.A.; Steele, J.F.; Knight, H.

The Arabidopsis Mediator complex subunits MED16, MED14 and MED2 regulate Mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes Thumbnail


Authors

P.A. Hemsley

C.H. Hurst

E. Kaliyadasa

R. Lamb

E.A. De Cothi

J.F. Steele



Abstract

The Mediator16 (MED16; formerly termed SENSITIVE TO FREEZING6 [SFR6]) subunit of the plant Mediator transcriptional coactivator complex regulates cold-responsive gene expression in Arabidopsis thaliana, acting downstream of the C-repeat binding factor (CBF) transcription factors to recruit the core Mediator complex to cold-regulated genes. Here, we use loss-of-function mutants to show that RNA polymerase II recruitment to CBF-responsive cold-regulated genes requires MED16, MED2, and MED14 subunits. Transcription of genes known to be regulated via CBFs binding to the C-repeat motif/drought-responsive element promoter motif requires all three Mediator subunits, as does cold acclimation–induced freezing tolerance. In addition, these three subunits are required for low temperature–induced expression of some other, but not all, cold-responsive genes, including genes that are not known targets of CBFs. Genes inducible by darkness also required MED16 but required a different combination of Mediator subunits for their expression than the genes induced by cold. Together, our data illustrate that plants control transcription of specific genes through the action of subsets of Mediator subunits; the specific combination defined by the nature of the stimulus but also by the identity of the gene induced.

Citation

Hemsley, P., Hurst, C., Kaliyadasa, E., Lamb, R., Knight, M., De Cothi, E., …Knight, H. (2014). The Arabidopsis Mediator complex subunits MED16, MED14 and MED2 regulate Mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes. The Plant Cell, 26(1), 465-484. https://doi.org/10.1105/tpc.113.117796

Journal Article Type Article
Acceptance Date Dec 17, 2013
Online Publication Date Jan 10, 2014
Publication Date Jan 10, 2014
Deposit Date Jan 16, 2014
Publicly Available Date Mar 29, 2024
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 26
Issue 1
Pages 465-484
DOI https://doi.org/10.1105/tpc.113.117796

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