Phosphate analogues in the dissection of mechanism

Korhonen, Heidi J.; Conway, Louis P.; Hodgson, David R.W.

doi:10.1016/j.cbpa.2014.05.001

Phosphate analogues in the dissection of mechanism

Korhonen, Heidi J.; Conway, Louis P.; Hodgson, David R.W.

Authors

Heidi J. Korhonen

Louis P. Conway

Professor David Hodgson d.r.w.hodgson@durham.ac.uk
Professor

Abstract

Phosphoryl group transfer is central to genetic replication, cellular signalling and many metabolic processes. Understanding the mechanisms of phosphorylation and phosphate ester and anhydride cleavage is key to efforts towards biotechnological and biomedical exploitation of phosphate-handling enzymes. Analogues of phosphate esters and anhydrides are indispensable tools, alongside protein mutagenesis and computational methods, for the dissection of phosphoryl transfer mechanisms. Hydrolysable and non-hydrolysable phosphate analogues have provided insight into the nature and sites of phosphoryl transfer processes. Kinetic isotope effects and crystallography using transition state analogues have painted more detailed pictures of transition states and how enzymes work to stabilise them.

Citation

Korhonen, H. J., Conway, L. P., & Hodgson, D. R. (2014). Phosphate analogues in the dissection of mechanism. Current Opinion in Chemical Biology, 21, 63-72. https://doi.org/10.1016/j.cbpa.2014.05.001

Journal Article Type	Article
Publication Date	Aug 1, 2014
Deposit Date	Mar 5, 2014
Publicly Available Date	Jun 16, 2014
Journal	Current Opinion in Chemical Biology
Print ISSN	1367-5931
Publisher	Elsevier
Peer Reviewed	Peer Reviewed
Volume	21
Pages	63-72
DOI	https://doi.org/10.1016/j.cbpa.2014.05.001