Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Carr, Rachel; Puckrin, Robert; McMahon, Brian K.; Pal, Robert; Parker, David; Pålsson, Lars-Olof

doi:10.1088/2050-6120/2/2/024007

Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Carr, Rachel; Puckrin, Robert; McMahon, Brian K.; Pal, Robert; Parker, David; Pålsson, Lars-Olof

Authors

Rachel Carr

Robert Puckrin

Brian K. McMahon

Professor Robert Pal robert.pal@durham.ac.uk
Professor

Professor David Parker david.parker@durham.ac.uk
Emeritus Professor

Dr Lars- Palsson lars-olof.palsson@durham.ac.uk
Associate Professor

Abstract

A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIII complexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, and the observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIII CPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.

Citation

Carr, R., Puckrin, R., McMahon, B. K., Pal, R., Parker, D., & Pålsson, L. (2014). Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes. Methods and Applications in Fluorescence, 2(2), Article 024007. https://doi.org/10.1088/2050-6120/2/2/024007

Journal Article Type	Article
Acceptance Date	Dec 18, 2013
Online Publication Date	Apr 9, 2014
Publication Date	Jun 1, 2014
Deposit Date	Jun 19, 2014
Publicly Available Date	Dec 9, 2014
Journal	Methods and Applications in Fluorescence
Publisher	IOP Publishing
Peer Reviewed	Peer Reviewed
Volume	2
Issue	2
Article Number	024007
DOI	https://doi.org/10.1088/2050-6120/2/2/024007

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