N.E. Ellot
Long-term effects of gestational nicone exposure and food-restriction on gene expression in the striatum of adolescent rats
Ellot, N.E.; Schneider, T.; Mill, J.; Schalkwyk, L.; Giovana, B.; Bizarro, L.; Stolerman, I.P.; Dempster, E.; Asherson, P.
Authors
T. Schneider
J. Mill
L. Schalkwyk
B. Giovana
L. Bizarro
I.P. Stolerman
E. Dempster
P. Asherson
Abstract
Gestational exposure to environmental toxins such as nicotine may result in detectable gene expression changes in later life. To investigate the direct toxic effects of prenatal nicotine exposure on later brain development, we have used transcriptomic analysis of striatal samples to identify gene expression differences between adolescent Lister Hooded rats exposed to nicotine in utero and controls. Using an additional group of animals matched for the reduced food intake experienced in the nicotine group, we were also able to assess the impact of imposed food-restriction on gene expression profiles. We found little evidence for a role of gestational nicotine exposure on altered gene expression in the striatum of adolescent offspring at a significance level of p<0.01 and |log2 fold change >0.5|, although we cannot exclude the possibility of nicotine-induced changes in other brain regions, or at other time points. We did, however, find marked gene expression differences in response to imposed food-restriction. Food-restriction resulted in significant group differences for a number of immediate early genes (IEGs) including Fos, Fosb, Fosl2, Arc, Junb, Nr4a1 and Nr4a3. These genes are associated with stress response pathways and therefore may reflect long-term effects of nutritional deprivation on the development of the stress system.
Citation
Ellot, N., Schneider, T., Mill, J., Schalkwyk, L., Giovana, B., Bizarro, L., …Asherson, P. (2014). Long-term effects of gestational nicone exposure and food-restriction on gene expression in the striatum of adolescent rats. PLoS ONE, 9(2), Article e88896. https://doi.org/10.1371/journal.pone.0088896
Journal Article Type | Article |
---|---|
Publication Date | Feb 19, 2014 |
Deposit Date | Jan 23, 2014 |
Publicly Available Date | Aug 21, 2014 |
Journal | PLoS ONE |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Issue | 2 |
Article Number | e88896 |
DOI | https://doi.org/10.1371/journal.pone.0088896 |
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Copyright Statement
© 2014 Ilott et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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