Metal Preferences and Metallation

Foster, Andrew W.; Osman, Deenah; Robinson, Nigel J.

doi:10.1074/jbc.r114.588145

Metal Preferences and Metallation

Foster, Andrew W.; Osman, Deenah; Robinson, Nigel J.

Authors

Andrew W. Foster

Deenah Osman

Professor Nigel Robinson nigel.robinson@durham.ac.uk
Professor

Abstract

The metal-binding preferences of most metalloproteins do not match their metal-requirements. Thus, metallation of an estimated 30% of metalloenzymes is aided by metal-delivery systems, with ~25% acquiring pre-assembled metal-cofactors. The remaining ~70% are presumed to compete for metals from buffered metal-pools. Metallation is further aided by maintaining the relative concentrations of these pools as an inverse function of the stabilities of the respective metal complexes. For example, magnesium enzymes always prefer to bind zinc and these metals dominate the metalloenzymes without metal-delivery systems. Therefore, the buffered concentration of zinc is held at least a million-fold below magnesium inside most cells.

Citation

Foster, A. W., Osman, D., & Robinson, N. J. (2014). Metal Preferences and Metallation. Journal of Biological Chemistry, 289(41), 28095-28103. https://doi.org/10.1074/jbc.r114.588145

Journal Article Type	Article
Online Publication Date	Aug 26, 2014
Publication Date	Oct 10, 2014
Deposit Date	Sep 11, 2014
Publicly Available Date	Sep 11, 2014
Journal	Journal of Biological Chemistry
Print ISSN	0021-9258
Electronic ISSN	1083-351X
Publisher	American Society for Biochemistry and Molecular Biology
Peer Reviewed	Peer Reviewed
Volume	289
Issue	41
Pages	28095-28103
DOI	https://doi.org/10.1074/jbc.r114.588145
Keywords	Copper, Iron, Manganese, Nickel, Zinc, Irving-Williams Series, Metal sensors, Metallochaperone, Metalloenzymes, Polydisperse, Buffer.
Public URL	https://durham-repository.worktribe.com/output/1454202
Related Public URLs	http://www.ncbi.nlm.nih.gov/pubmed/25160626

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Advance online version © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License applies to Author Choice Articles.