EuroTracker® dyes : design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging

Butler, S.J.; Delbianco, M.; Lamarque, L.; McMahon, B.K.; Neil, E.R.; Pal, R.; Parker, D.; Walton, J.W.; Zwier, J.

doi:10.1039/c4dt02785j

EuroTracker® dyes : design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging

Butler, S.J.; Delbianco, M.; Lamarque, L.; McMahon, B.K.; Neil, E.R.; Pal, R.; Parker, D.; Walton, J.W.; Zwier, J.

Authors

S.J. Butler

M. Delbianco

L. Lamarque

B.K. McMahon

E.R. Neil

Professor Robert Pal robert.pal@durham.ac.uk
Professor

Professor David Parker david.parker@durham.ac.uk
Emeritus Professor

Dr James Walton james.walton@durham.ac.uk
Associate Professor

J. Zwier

Abstract

The development of the brightest luminescent europium(III) complexes is traced, including analysis of the C3-symmetric core complex based on a functionalized triazacyclononane and identification of the most suitable strongly absorbing chromophore. Strategies for the synthesis of the complexes, including enantiopure analogues, are outlined and opportunities for applications in time-resolved microscopy and spectral imaging emphasised. Practicable examples are introduced, including selective organelle staining for cellular optical imaging at 65 nm resolution and the development of new bioassays using time-resolved FRET methods.

Citation

Butler, S., Delbianco, M., Lamarque, L., McMahon, B., Neil, E., Pal, R., …Zwier, J. (2015). EuroTracker® dyes : design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging. Dalton Transactions, 44(11), 4791-4803. https://doi.org/10.1039/c4dt02785j

Journal Article Type	Article
Acceptance Date	Oct 10, 2014
Online Publication Date	Oct 13, 2014
Publication Date	Mar 21, 2015
Deposit Date	Nov 14, 2014
Publicly Available Date	Nov 20, 2014
Journal	Dalton Transactions
Print ISSN	1477-9226
Electronic ISSN	1477-9234
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	44
Issue	11
Pages	4791-4803
DOI	https://doi.org/10.1039/c4dt02785j