Bright, highly water soluble triazacyclononane europium complexes to detect ligand binding with time-resolved FRET microscopy

Delbianco, M.; Sadovnikova, V.; Bourrier, E.; Mathis, G.; Lamarque, L.; Zwier, J.M.; Parker, D.

doi:10.1002/anie.201406632

Bright, highly water soluble triazacyclononane europium complexes to detect ligand binding with time-resolved FRET microscopy

Delbianco, M.; Sadovnikova, V.; Bourrier, E.; Mathis, G.; Lamarque, L.; Zwier, J.M.; Parker, D.

Authors

M. Delbianco

V. Sadovnikova

E. Bourrier

G. Mathis

L. Lamarque

J.M. Zwier

Professor David Parker david.parker@durham.ac.uk
Emeritus Professor

Abstract

Luminescent europium complexes are used in a broad range of applications as a result of their particular emissive properties. The synthesis and application of bright, highly water-soluble, and negatively charged sulfonic- or carboxylic acid derivatives of para-substituted aryl–alkynyl triazacyclononane complexes are described. Introduction of the charged solubilizing moieties suppresses cellular uptake or adsorption to living cells making them applicable for labeling and performing assays on membrane receptors. These europium complexes are applied to monitor fluorescent ligand binding on cell-surface proteins with time-resolved Förster resonance energy transfer (TR-FRET) assays in plate-based format and using TR-FRET microscopy.

Citation

Delbianco, M., Sadovnikova, V., Bourrier, E., Mathis, G., Lamarque, L., Zwier, J., & Parker, D. (2014). Bright, highly water soluble triazacyclononane europium complexes to detect ligand binding with time-resolved FRET microscopy. Angewandte Chemie International Edition, 53(40), 10718-10722. https://doi.org/10.1002/anie.201406632

Journal Article Type	Article
Online Publication Date	Sep 26, 2014
Publication Date	Sep 26, 2014
Deposit Date	Nov 14, 2014
Publicly Available Date	Dec 1, 2014
Journal	Angewandte Chemie International Edition
Print ISSN	1433-7851
Electronic ISSN	1521-3773
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	53
Issue	40
Pages	10718-10722
DOI	https://doi.org/10.1002/anie.201406632
Keywords	Europium, G protein-coupled receptors, Imaging agents, Macrocycles, Time-resolved FRET.

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Copyright Statement
This is the peer reviewed version of the following article: Delbianco, M., Sadovnikova, V., Bourrier, E., Mathis, G., Lamarque, L., Zwier, J. M. and Parker, D. (2014), Bright, Highly Water-Soluble Triazacyclononane Europium Complexes To Detect Ligand Binding with Time-Resolved FRET Microscopy. Angewandte Chemie International Edition, 53 (40): 10718-10722, which has been published in final form at http://dx.doi.org/10.1002/anie.201406632. This article may be used for non-commercial purposes in accordance With Wiley-VCH Terms and Conditions for self-archiving.