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Dysferlin regulates cell membrane repair by facilitating injury-triggered acid sphingomyelinase secretion.

Defour, A. and van der Meulen, J.H. and Bhat, R. and Bigot, A. and Bashir, R. and Nagaraju, K. and Jaiswal, J.K. (2014) 'Dysferlin regulates cell membrane repair by facilitating injury-triggered acid sphingomyelinase secretion.', Cell death and disease., 5 (6). e1306.

Abstract

Dysferlin deficiency compromises the repair of injured muscle, but the underlying cellular mechanism remains elusive. To study this phenomenon, we have developed mouse and human myoblast models for dysferlinopathy. These dysferlinopathic myoblasts undergo normal differentiation but have a deficit in their ability to repair focal injury to their cell membrane. Imaging cells undergoing repair showed that dysferlin-deficit decreased the number of lysosomes present at the cell membrane, resulting in a delay and reduction in injury-triggered lysosomal exocytosis. We find repair of injured cells does not involve formation of intracellular membrane patch through lysosome–lysosome fusion; instead, individual lysosomes fuse with the injured cell membrane, releasing acid sphingomyelinase (ASM). ASM secretion was reduced in injured dysferlinopathic cells, and acute treatment with sphingomyelinase restored the repair ability of dysferlinopathic myoblasts and myofibers. Our results provide the mechanism for dysferlin-mediated repair of skeletal muscle sarcolemma and identify ASM as a potential therapy for dysferlinopathy.

Item Type:Article
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Status:Peer-reviewed
Publisher Web site:http://dx.doi.org/10.1038/cddis.2014.272
Publisher statement:Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/.
Record Created:13 Apr 2015 10:20
Last Modified:13 Apr 2015 13:06

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