Distinct shifts in microbiota composition during Drosophila aging impair intestinal function and drive mortality

Clark, R.I.; Salazar, A.; Yamada, R.; Fitz-Gibbon, S.; Morselli, M.; Alcaraz, J.; Rana, A.; Rera, M.; Pellegrini, M.; Ja, W.W.; Walker, D.W.

doi:10.1016/j.celrep.2015.08.004

Distinct shifts in microbiota composition during Drosophila aging impair intestinal function and drive mortality

Clark, R.I.; Salazar, A.; Yamada, R.; Fitz-Gibbon, S.; Morselli, M.; Alcaraz, J.; Rana, A.; Rera, M.; Pellegrini, M.; Ja, W.W.; Walker, D.W.

Authors

Dr Rebecca Clark rebecca.clark2@durham.ac.uk
Associate Professor

A. Salazar

R. Yamada

S. Fitz-Gibbon

M. Morselli

J. Alcaraz

A. Rana

M. Rera

M. Pellegrini

W.W. Ja

D.W. Walker

Abstract

Alterations in the composition of the intestinal microbiota have been correlated with aging and measures of frailty in the elderly. However, the relationships between microbial dynamics, age-related changes in intestinal physiology, and organismal health remain poorly understood. Here, we show that dysbiosis of the intestinal microbiota, characterized by an expansion of the Gammaproteobacteria, is tightly linked to age-onset intestinal barrier dysfunction in Drosophila. Indeed, alterations in the microbiota precede and predict the onset of intestinal barrier dysfunction in aged flies. Changes in microbial composition occurring prior to intestinal barrier dysfunction contribute to changes in excretory function and immune gene activation in the aging intestine. In addition, we show that a distinct shift in microbiota composition follows intestinal barrier dysfunction, leading to systemic immune activation and organismal death. Our results indicate that alterations in microbiota dynamics could contribute to and also predict varying rates of health decline during aging in mammals.

Citation

Clark, R., Salazar, A., Yamada, R., Fitz-Gibbon, S., Morselli, M., Alcaraz, J., …Walker, D. (2015). Distinct shifts in microbiota composition during Drosophila aging impair intestinal function and drive mortality. Cell Reports, 12(10), 1656-1667. https://doi.org/10.1016/j.celrep.2015.08.004

Journal Article Type	Article
Acceptance Date	Jul 31, 2015
Online Publication Date	Aug 28, 2015
Publication Date	Sep 8, 2015
Deposit Date	Aug 28, 2015
Publicly Available Date	Sep 1, 2015
Journal	Cell Reports
Print ISSN	2211-1247
Publisher	Cell Press
Peer Reviewed	Peer Reviewed
Volume	12
Issue	10
Pages	1656-1667
DOI	https://doi.org/10.1016/j.celrep.2015.08.004