Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy

Chansawang, Narin; Obara, Boguslaw; Geider, Richard J.; Laissue, Pierre Philippe

doi:10.1007/8623_2015_142

Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy

Chansawang, Narin; Obara, Boguslaw; Geider, Richard J.; Laissue, Pierre Philippe

Authors

Narin Chansawang

Boguslaw Obara

Richard J. Geider

Pierre Philippe Laissue

Contributors

Terry J. McGenity
Editor

Kenneth N. Timmis
Editor

Balbina Nogales Fernández
Editor

Abstract

Fluorescence microscopy and digital imaging allow the selective visualisation and quantification of cellular components and can convey research findings in an appealing and intuitive way. These techniques are regularly used in biomedical research laboratories, but have less widespread application in marine sciences. We present here an approach to label and volumetrically quantify neutral lipids, chloroplasts, DNA and cell volumes in microalgae. Using fluorescence microscopy techniques on “turn-key” systems commonly available to environmental research labs, imaging facilities or accessible groups in other disciplines ensure that this approach can be widely reproduced.

Citation

Chansawang, N., Obara, B., Geider, R. J., & Laissue, P. P. (2015). Three-dimensional visualization and quantification of lipids in microalgae using confocal laser scanning microscopy. In T. J. McGenity, K. N. Timmis, & B. Nogales Fernández (Eds.), Hydrocarbon and lipid microbiology protocols : ultrastructure and imaging (145-161). Springer Verlag. https://doi.org/10.1007/8623_2015_142

Online Publication Date	Sep 8, 2015
Publication Date	Sep 8, 2015
Deposit Date	Oct 12, 2015
Publisher	Springer Verlag
Pages	145-161
Series Title	Springer protocols handbooks
Book Title	Hydrocarbon and lipid microbiology protocols : ultrastructure and imaging.
ISBN	9783662491324
DOI	https://doi.org/10.1007/8623_2015_142