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SUMOylation of phytochrome-B negatively regulates light-induced signaling in Arabidopsis thaliana.

Sadanandom, A. and Ádám, É. and Orosa, B. and Viczián, A. and Klose, C. and Zhang, C. and Josse, E.M. and Kozma-Bognár, L. and Nagy, F. (2015) 'SUMOylation of phytochrome-B negatively regulates light-induced signaling in Arabidopsis thaliana.', Proceedings of the National Academy of Sciences of the United States of America., 112 (35). pp. 11108-11113.

Abstract

The red/far red light absorbing photoreceptor phytochrome-B (phyB) cycles between the biologically inactive (Pr, λmax, 660 nm) and active (Pfr; λmax, 730 nm) forms and functions as a light quality and quantity controlled switch to regulate photomorphogenesis in Arabidopsis. At the molecular level, phyB interacts in a conformation-dependent fashion with a battery of downstream regulatory proteins, including PHYTOCHROME INTERACTING FACTOR transcription factors, and by modulating their activity/abundance, it alters expression patterns of genes underlying photomorphogenesis. Here we report that the small ubiquitin-like modifier (SUMO) is conjugated (SUMOylation) to the C terminus of phyB; the accumulation of SUMOylated phyB is enhanced by red light and displays a diurnal pattern in plants grown under light/dark cycles. Our data demonstrate that (i) transgenic plants expressing the mutant phyBLys996Arg-YFP photoreceptor are hypersensitive to red light, (ii) light-induced SUMOylation of the mutant phyB is drastically decreased compared with phyB-YFP, and (iii) SUMOylation of phyB inhibits binding of PHYTOCHROME INTERACTING FACTOR 5 to phyB Pfr. In addition, we show that OVERLY TOLERANT TO SALT 1 (OTS1) de-SUMOylates phyB in vitro, it interacts with phyB in vivo, and the ots1/ots2 mutant is hyposensitive to red light. Taken together, we conclude that SUMOylation of phyB negatively regulates light signaling and it is mediated, at least partly, by the action of OTS SUMO proteases.

Item Type:Article
Keywords:Photoreceptor, Phytochrome, Sumoylation, Signaling, Photomorphogenesis.
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1073/pnas.1415260112
Record Created:02 Nov 2015 15:50
Last Modified:03 Nov 2015 09:20

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