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Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope

Maia, A.M.A.; Longbottom, C.; Gomes, A.S.L.; Girkin, J.M.

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Authors

A.M.A. Maia

C. Longbottom

A.S.L. Gomes



Abstract

The aim of this study was to evaluate the erosion-inhibiting effect of two toothpastes on the development of erosion-like lesions, by a confocal laser scanning microscope (CLSM). Forty human enamel blocks were divided into five groups (n = 8), in accordance to evaluate the GC MI Paste Plus and Oral B with stannous fluoride, applied as slurries and associated with toothbrush. Specimens were submitted to an erosion challenge from citric acid (0.5%, pH = 2.8), for 5 min, six times a day, alternating in artificial saliva immersions. Reference group was not exposed to treatment. Part of specimens (Groups 02 and 03) was exposed twice daily just to slurries, for 2 min, therefore specimens from Groups 04 and 05 were also abraded, for 30 s. The enamel surfaces were morphological characterized using CLSM images, with mineral loss being measured using the resulting 3D images referenced to an un-challenged portion of the sample. Step values were compared using the one-way ANOVA test. CLSM was shown to be a viable, noncontact, and simple technique to characterize eroded surfaces. The statistical difference in the step size was significant between the groups (P = 0.001) and using multiple comparisons a statistically significant protective effect of toothpastes was shown when these were applied as slurries. Although groups submitted to tooth brush showed mineral loss similar to reference control group, due to the damages of abrasion associated

Citation

Maia, A., Longbottom, C., Gomes, A., & Girkin, J. (2014). Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope. Microscopy Research and Technique, 77(6), 439-445. https://doi.org/10.1002/jemt.22364

Journal Article Type Article
Acceptance Date Mar 21, 2014
Publication Date Jun 1, 2014
Deposit Date Nov 18, 2015
Publicly Available Date Mar 29, 2024
Journal Microscopy Research and Technique
Print ISSN 1059-910X
Electronic ISSN 1097-0029
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 77
Issue 6
Pages 439-445
DOI https://doi.org/10.1002/jemt.22364
Keywords Fluoride, Tooth erosion, Enamel surface.

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Copyright Statement
This is the accepted version of the following article: Maia, A. M. A., Longbottom, C., Gomes, A. S. L. and Girkin, J. M. (2014), Enamel erosion and prevention efficacy characterized by confocal laser scanning microscope. Microscopy Research and Technique, 77(6): 439-445, which has been published in final form at http://dx.doi.org/10.1002/jemt.22364. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.





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