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Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection

Lawton, P.F.; Saunter, C.D.; Girkin, J.M.

Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection Thumbnail


Authors

C.D. Saunter



Contributors

Bonnie L. Gray
Editor

Holger Becker
Editor

Abstract

Since their invention by Ashkin optical tweezers have demonstrated their ability and versatility as a non-invasive tool for micromanipulation. One of the most useful additions to the basic optical tweezers system is micro-Raman spectroscopy, which permits highly sensitive analysis of single cells or particles. We report on the development of a dual laser system combining two spatial light modulators to holographically manipulate multiple traps (at 1064nm) whilst undertaking Raman spectroscopy using a 532nm laser. We can thus simultaneously trap multiple particles and record their Raman spectra, without perturbing the trapping system. The dual beam system is built around micro-fluidic channels where crystallisation of calcium carbonate occurs on polymethylmethacrylate (PMMA) beads. The setup is designed to simulate at a microscopic level the reactions that occur on items in a dishwasher, where permanent filming of calcium carbonate on drinking glasses is a problem. Our system allows us to monitor crystal growth on trapped particles in which the Raman spectrum and changes in movement of the bead are recorded. Due to the expected low level of crystallisation on the bead surfaces this allows us to obtain results quickly and with high sensitivity. The long term goal is to study the development of filming on samples in-situ with the microfluidic system acting as a model dishwasher.

Citation

Lawton, P., Saunter, C., & Girkin, J. (2014). Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection. In B. L. Gray, & H. Becker (Eds.), Proceedings of SPIE. https://doi.org/10.1117/12.2037022

Conference Name Microfluidics, BioMEMS, and Medical Microsystems XII
Conference Location San Francisco, California, United States
Acceptance Date Mar 2, 2014
Publication Date Mar 1, 2014
Deposit Date Nov 18, 2015
Publicly Available Date Mar 29, 2024
Volume 8976
Series Number 8976
Series ISSN 0277-786X,1996-756X
Book Title Proceedings of SPIE.
DOI https://doi.org/10.1117/12.2037022
Keywords Optical tweezers, Raman spectroscopy, Crystallization, Motion detection.
Public URL https://durham-repository.worktribe.com/output/1153035

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Published Conference Proceeding (938 Kb)
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Copyright Statement
Copyright 2014 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Lawton, P. F. and Saunter, C. D. and Girkin, J. M. (2014) 'Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection.', in Proceedings of SPIE edited by B.L. Gray and H. Becker Volume 8976, Article 89760V, 2014.





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