Robust NMR water signal suppression for demanding analytical applications

Aguilar, Juan A.; Kenwright, Simon J.

doi:10.1039/c5an02121a

Robust NMR water signal suppression for demanding analytical applications

Aguilar, Juan A.; Kenwright, Simon J.

Authors

Juan A. Aguilar

Simon J. Kenwright

Contributors

Dr Juan Aguilar Malavia j.a.aguilar@durham.ac.uk
Other

Abstract

We describe the design and application of robust, general-purpose water signal suppression pulse sequences well suited to chemometric work. Such pulse sequences need to deal well with pulse mis-calibrations, radiation damping, chemical exchange, and the presence of sample inhomogeneities, as well as with significant variations in sample characteristics such as pH, ionic strength, relaxation characteristics and molecular weight. Of course, such pulse sequences should produce un-distorted lineshapes and baselines and work well both under automation and in the hands of non-experts. As an example, one such pulse sequences, Robust-5, will be presented. This new pulse sequence meets those criteria and is able to reduce a 50 M proteo water signal down to a 0.9 mM level, without fine tuning, and under automation, and it is therefore well suited to the most demanding of analytical applications.

Citation

Aguilar, J. A., & Kenwright, S. J. (2016). Robust NMR water signal suppression for demanding analytical applications. Analyst, 141(1), 236-242. https://doi.org/10.1039/c5an02121a

Journal Article Type	Article
Acceptance Date	Nov 6, 2015
Online Publication Date	Nov 18, 2015
Publication Date	Jan 7, 2016
Deposit Date	Dec 8, 2015
Publicly Available Date	Nov 6, 2016
Journal	Analyst
Print ISSN	0003-2654
Electronic ISSN	1364-5528
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Volume	141
Issue	1
Pages	236-242
DOI	https://doi.org/10.1039/c5an02121a