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Excited State Dynamics of the Isolated Green Fluorescent Protein Chromophore Anion Following UV Excitation

West, C.W.; Bull, J.N.; Hudson, A.S.; Cobb, S.L.; Verlet, J.R.R.

Excited State Dynamics of the Isolated Green Fluorescent Protein Chromophore Anion Following UV Excitation Thumbnail


Authors

C.W. West

J.N. Bull

A.S. Hudson

S.L. Cobb



Abstract

A combined frequency-, angle-, and time-resolved photoelectron spectroscopy study is used to unravel the excited state dynamics following UV excitation of the isolated anionic chromophore of the green fluorescent protein (GFP). The optically bright S3 state, which is populated for hv > 3.7 eV, is shown to decay predominantly by internal conversion to the S2 state that in turn autodetaches to the neutral ground state. For hv > 4.1 eV, a new and favorable autodetachment channel from the S2 state becomes available, which leads to the formation of the neutral in an excited state. The results indicate that the UV excited state dynamics of the GFP chromophore involve a number of strongly coupled excited states.

Citation

West, C., Bull, J., Hudson, A., Cobb, S., & Verlet, J. (2015). Excited State Dynamics of the Isolated Green Fluorescent Protein Chromophore Anion Following UV Excitation. Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry), 119(10), 3982-3987. https://doi.org/10.1021/acs.jpcb.5b01432

Journal Article Type Article
Acceptance Date Feb 11, 2015
Publication Date Mar 12, 2015
Deposit Date Jun 4, 2015
Publicly Available Date Mar 29, 2024
Journal Journal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry)
Print ISSN 1520-6106
Electronic ISSN 1520-5207
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 119
Issue 10
Pages 3982-3987
DOI https://doi.org/10.1021/acs.jpcb.5b01432

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Copyright Statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcb.5b01432.




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