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Photoelectron spectroscopy of the model GFP chromophore anion

Horke, D.A.; Verlet, J.R.R.

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Authors

D.A. Horke



Abstract

A photoelectron spectroscopy study of the anionic model chromophore of the green fluorescent protein is presented. From the photoelectron spectra taken at 3.496 eV, 4.62 eV, and 6.15 eV the vertical and adiabatic detachment energies are determined to be 2.8 ± 0.1 eV and 2.6 ± 0.2 eV, respectively. The vertical detachment energy is higher than the S1 ← S0 absorption maximum (2.57 eV) and indicates that the S1 state is bound with respect to electron detachment in the Franck–Condon region. The photoelectron spectrum taken at 6.15 eV, together with TD-DFT calculations, are used to assign a number of excited states in the neutral radical that correspond to electron loss from occupied orbitals in the anion. The photoelectron spectrum at 2.58 eV shows evidence for electrons formed by thermionic emission, suggesting that internal conversion is the dominant relaxation pathway following S1 ← S0 excitation.

Citation

Horke, D., & Verlet, J. (2012). Photoelectron spectroscopy of the model GFP chromophore anion. Physical Chemistry Chemical Physics, 14(24), 8511-8515. https://doi.org/10.1039/c2cp40880e

Journal Article Type Article
Acceptance Date May 4, 2012
Publication Date Jun 28, 2012
Deposit Date Jan 28, 2016
Publicly Available Date Mar 28, 2024
Journal Physical Chemistry Chemical Physics
Print ISSN 1463-9076
Electronic ISSN 1463-9084
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 14
Issue 24
Pages 8511-8515
DOI https://doi.org/10.1039/c2cp40880e

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