Sorcha Finnegan
Protein expression profiling during chick retinal maturation: a proteomics-based approach
Finnegan, Sorcha; Robson, Joanne L.; Wylie, Mildred; Healy, Adrienne; Stitt, Alan W.; Curry, William J.
Authors
Joanne Robson j.l.robson@durham.ac.uk
Senior Experimental Officer
Mildred Wylie
Adrienne Healy
Alan W. Stitt
William J. Curry
Abstract
Background The underlying pathways that drive retinal neurogenesis and synaptogenesis are still relatively poorly understood. Protein expression analysis can provide direct insight into these complex developmental processes. The aim of this study was therefore to employ proteomic analysis to study the developing chick retina throughout embryonic (E) development commencing at day 12 through 13, 17, 19 and post-hatch (P) 1 and 33 days. Results 2D proteomic and mass spectrometric analysis detected an average of 1514 spots per gel with 15 spots demonstrating either modulation or constitutive expression identified via MS. Proteins identified included alpha and beta-tubulin, alpha enolase, B-creatine kinase, gamma-actin, platelet-activating factor (PAF), PREDICTED: similar to TGF-beta interacting protein 1, capping protein (actin filament muscle Z line), nucleophosmin 1 (NPM1), dimethylarginine dimethylaminohydrolase, triosphoaphate isomerase, DJ1, stathmin, fatty acid binding protein 7 (FABP7/B-FABP), beta-synuclein and enhancer of rudimentary homologue. Conclusion This study builds upon previous proteomic investigations of retinal development and represents the addition of a unique data set to those previously reported. Based on reported bioactivity some of the identified proteins are most likely to be important to normal retinal development in the chick. Continued analysis of the dynamic protein populations present at the early stages and throughout retinal development will increase our understanding of the molecular events underpinning retinogenesis.
Citation
Finnegan, S., Robson, J. L., Wylie, M., Healy, A., Stitt, A. W., & Curry, W. J. (2008). Protein expression profiling during chick retinal maturation: a proteomics-based approach. Proteome Science, 6(1), Article 34. https://doi.org/10.1186/1477-5956-6-34
Journal Article Type | Article |
---|---|
Publication Date | Dec 10, 2008 |
Deposit Date | Jan 27, 2014 |
Publicly Available Date | Apr 21, 2016 |
Journal | Proteome Science |
Publisher | BioMed Central |
Peer Reviewed | Peer Reviewed |
Volume | 6 |
Issue | 1 |
Article Number | 34 |
DOI | https://doi.org/10.1186/1477-5956-6-34 |
Files
Published Journal Article
(2.4 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
© Finnegan et al; licensee BioMed Central Ltd. 2008 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
You might also like
Expansion Microscopy of Plant Cells (PlantExM)
(2023)
Book Chapter
Proteomic profiling of the retinal dysplasia and degeneration chick
retina
(2010)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search