Skip to main content

Research Repository

Advanced Search

GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis

Schorr-Lenz, A.; Alves, J.; Hence, N.A.; Seidel, P.M.; Benham, A.M.; Bustamante-Filho, I.C.

GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis Thumbnail


Authors

A. Schorr-Lenz

J. Alves

N.A. Hence

P.M. Seidel

I.C. Bustamante-Filho



Abstract

Hypogonadism is defined as the inadequate gonadal production of testosterone. Low serum testosterone leads to infertility by impairing spermatogenesis and reducing sperm count, however, the impact of hypogonadism in epididymal sperm maturation is poorly understood. From the testis, spermatozoa are transported into the epididymis where they find a specific microenvironment composed of a complex mixture of proteins that facilitate sperm storage and maturation. Inside the epididymal ductule, spermatozoa undergo several changes, resulting in their becoming capable of fertilizing eggs. Protein disulfide isomerases (PDIs) are known to participate in the folding and assembly of secreted proteins in the endoplasmic reticulum. However, little is known about the control and function of PDIs in the testis and epididymis, particularly during male development. The aim of this work was to compare the expression and distribution of PDI and PDIA3 (ERp57) in the testis and epididymis of healthy and GnRH-immunized boars. We detected higher amounts of PDIA3 and PDI in sperm preparations and fluid from the proximal regions of the epididymis of healthy boars. However, we observed an increase in PDIA3 expression in the testis and cauda epididymis in the immunocastrated group. GnRH-immunized boars showed a marked increase in PDI content in cauda spermatozoa and fluid, indicating a possible endocrine dysregulation of PDI. The results of our study suggest that PDIs are associated with epididymal sperm maturation and may be attractive candidates for monitoring male fertility.

Citation

Schorr-Lenz, A., Alves, J., Hence, N., Seidel, P., Benham, A., & Bustamante-Filho, I. (2016). GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis. Andrology, 4(5), 957-963. https://doi.org/10.1111/andr.12205

Journal Article Type Article
Acceptance Date Apr 2, 2016
Online Publication Date Jun 20, 2016
Publication Date Sep 1, 2016
Deposit Date Jun 23, 2016
Publicly Available Date Mar 29, 2024
Journal Andrology
Print ISSN 2047-2919
Electronic ISSN 2047-2927
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 4
Issue 5
Pages 957-963
DOI https://doi.org/10.1111/andr.12205

Files

Accepted Journal Article (1.6 Mb)
PDF

Copyright Statement
This is the accepted version of the following article: Schorr-Lenz, A. M., Alves, J., Henckes, N. A. C., Seibel, P. M., Benham, A. M. and Bustamante-Filho, I. C. (2016), GnRH immunization alters the expression and distribution of protein disulfide isomerases in the epididymis. Andrology, 4(5): 957-963, which has been published in final form at http://dx.doi.org/10.1111/andr.12205. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.





You might also like



Downloadable Citations