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Identification of MEDIATOR16 as the Arabidopsis COBRA suppressor, MONGOOSE1

Sorek, N.; Szemenyei, H.; Sorek, H.; Landers, A.; Knight, H.; Bauer, S.; Wemmer, D.E.; Somerville, D.R.

Identification of MEDIATOR16 as the Arabidopsis COBRA suppressor, MONGOOSE1 Thumbnail


Authors

N. Sorek

H. Szemenyei

H. Sorek

A. Landers

S. Bauer

D.E. Wemmer

D.R. Somerville



Abstract

We performed a screen for genetic suppressors of cobra, an Arabidopsis mutant with defects in cellulose formation and an increased ratio of unesterified/esterified pectin. We identified a suppressor named mongoose1 (mon1) that suppressed the growth defects of cobra, partially restored cellulose levels, and restored the esterification ratio of pectin to wild-type levels. mon1 was mapped to the MEDIATOR16 (MED16) locus, a tail mediator subunit, also known as SENSITIVE TO FREEZING6 (SFR6). When separated from the cobra mutation, mutations in MED16 caused resistance to cellulose biosynthesis inhibitors, consistent with their ability to suppress the cobra cellulose deficiency. Transcriptome analysis revealed that a number of cell wall genes are misregulated in med16 mutants. Two of these genes encode pectin methylesterase inhibitors, which, when ectopically expressed, partially suppressed the cobra phenotype. This suggests that cellulose biosynthesis can be affected by the esterification levels of pectin, possibly through modifying cell wall integrity or the interaction of pectin and cellulose.

Citation

Sorek, N., Szemenyei, H., Sorek, H., Landers, A., Knight, H., Bauer, S., …Somerville, D. (2015). Identification of MEDIATOR16 as the Arabidopsis COBRA suppressor, MONGOOSE1. Proceedings of the National Academy of Sciences, 112(52), 16048-16053. https://doi.org/10.1073/pnas.1521675112

Journal Article Type Article
Acceptance Date Dec 11, 2015
Online Publication Date Dec 11, 2015
Publication Date Dec 29, 2015
Deposit Date Jan 5, 2016
Publicly Available Date Mar 29, 2024
Journal Proceedings of the National Academy of Sciences
Print ISSN 0027-8424
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 112
Issue 52
Pages 16048-16053
DOI https://doi.org/10.1073/pnas.1521675112

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