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Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell Type Proliferation and Differentiation

de Lucas, Miguel; Pu, Li; Turco, Gina Marie; Gaudinier, Allie; Marao, Ana Karina; Hiroshima, Hirofumi; Kim, Dahae; Ron, Mily; Sugimoto, Keiko; Roudier, Francois M.; Brady, Siobhan M.

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Authors

Li Pu

Gina Marie Turco

Allie Gaudinier

Ana Karina Marao

Hirofumi Hiroshima

Dahae Kim

Mily Ron

Keiko Sugimoto

Francois M. Roudier

Siobhan M. Brady



Abstract

Spatiotemporal regulation of transcription is fine-tuned at multiple levels, including chromatin compaction. Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation of Histone 3 at lysine 27 (H3K27me3), which is the hallmark of a repressive chromatin state. Multiple PRC2 complexes have been reported in Arabidopsis thaliana to control the expression of genes involved in developmental transitions and maintenance of organ identity. Here, we show that PRC2 member genes display complex spatiotemporal gene expression patterns and function in root meristem and vascular cell proliferation and specification. Furthermore, PRC2 gene expression patterns correspond with vascular and non-vascular tissue-specific H3K27me3-marked genes. This tissue-specific repression via H3K27me3 regulates the balance between cell proliferation and differentiation. Using enhanced yeast-one-hybrid analysis, upstream regulators of the PRC2 member genes are identified, and genetic analysis demonstrates that transcriptional regulation of some PRC2 genes plays an important role in determining PRC2 spatiotemporal activity within a developing organ.

Citation

de Lucas, M., Pu, L., Turco, G. M., Gaudinier, A., Marao, A. K., Hiroshima, H., …Brady, S. M. (2016). Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell Type Proliferation and Differentiation. The Plant Cell, 28(10), 2616-2631. https://doi.org/10.1105/tpc.15.00744

Journal Article Type Article
Acceptance Date Sep 14, 2016
Online Publication Date Sep 20, 2016
Publication Date Oct 1, 2016
Deposit Date Sep 26, 2016
Publicly Available Date Mar 29, 2024
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 28
Issue 10
Pages 2616-2631
DOI https://doi.org/10.1105/tpc.15.00744

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Published Journal Article (Advance online version) (1.7 Mb)
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Copyright Statement
Advance online version Copyright American Society of Plant Biologists.


Published Journal Article (Final published version) (2.6 Mb)
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Final published version




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