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Induced europium CPL for the selective signalling of phosphorylated amino-acids and O-phosphorylated hexapeptides.

Neil, E.R. and Pal, R. and Fox, M.A. and Parker, D. (2016) 'Induced europium CPL for the selective signalling of phosphorylated amino-acids and O-phosphorylated hexapeptides.', Dalton transactions., 45 (20). pp. 8355-8366.

Abstract

Two bright, europium(III) complexes based on an achiral heptadentate triazacyclononane ligand bearing two strongly absorbing chromophores have been evaluated for the selective emission and CPL signalling of various chiral O-phosphono-anions. Binding of O-phosphono-Ser and Thr gives rise to a strong induced CPL signature and a favoured Δ complex configuration is adopted. A similarly large induced CPL signal arises when [Eu·L1]2+ binds to lysophosphatidic acid (LPA), where the strong binding (log K 5.25 (295 K)) in methanol allowed its detection over the range 5 to 40 μM. Strong and chemoselective binding to the phosphorylated amino-acid residues was also observed with a set of four structurally related hexapeptides: in one case, the sign of the gem value in the ΔJ = 1 transition allowed differentiation between the binding to O-P-Ser and O-P-Tyr residues.

Item Type:Article
Full text:(AM) Accepted Manuscript
Available under License - Creative Commons Attribution Non-commercial.
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1039/C6DT01212D
Publisher statement:This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Date accepted:19 April 2016
Date deposited:03 March 2017
Date of first online publication:20 April 2016
Date first made open access:No date available

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