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DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus

Cooper, L.P.; Roberts, G.A.; White, J.H.; Luyten, Y.A.; Bower, E.K.M.; Morgan, R.D.; Roberts, R.J.; Lindsay, J.A.; Dryden, D.T.F.

DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus Thumbnail


Authors

L.P. Cooper

G.A. Roberts

J.H. White

Y.A. Luyten

E.K.M. Bower

R.D. Morgan

R.J. Roberts

J.A. Lindsay



Abstract

Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction–modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this ‘untransformable’ bacterium.

Citation

Cooper, L., Roberts, G., White, J., Luyten, Y., Bower, E., Morgan, R., …Dryden, D. (2017). DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus. Nucleic Acids Research, 45(6), 3395-3406. https://doi.org/10.1093/nar/gkx067

Journal Article Type Article
Acceptance Date Jan 20, 2017
Online Publication Date Feb 9, 2017
Publication Date Apr 7, 2017
Deposit Date Mar 8, 2017
Publicly Available Date Mar 15, 2017
Journal Nucleic Acids Research
Print ISSN 0305-1048
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 45
Issue 6
Pages 3395-3406
DOI https://doi.org/10.1093/nar/gkx067

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Advance online version © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which
permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.





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