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Growth rate control of flagellar assembly in Escherichia coli strain RP437

Sim, Martin; Koirala, Santosh; Picton, David; Strahl, Henrik; Hoskisson, Paul A.; Rao, Christopher V.; Gillespie, Colin S.; Aldridge, Phillip D.

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Authors

Martin Sim

Santosh Koirala

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David Picton david.m.picton@durham.ac.uk
PGR Student Doctor of Philosophy

Henrik Strahl

Paul A. Hoskisson

Christopher V. Rao

Colin S. Gillespie

Phillip D. Aldridge



Abstract

The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a systematic study has not yet been described under controlled growth conditions. Here, we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-state chemostat cultures where we could precisely control the cell growth-rate. Our results demonstrate that flagellar abundance correlates with growth rate, where faster growing cells produce more flagella. They also demonstrate that this growth-rate dependent control occurs through the expression of the flagellar master regulator, FlhD4C2. Collectively, our results demonstrate that motility is intimately coupled to the growth-rate of the cell.

Citation

Sim, M., Koirala, S., Picton, D., Strahl, H., Hoskisson, P. A., Rao, C. V., …Aldridge, P. D. (2017). Growth rate control of flagellar assembly in Escherichia coli strain RP437. Scientific Reports, 7, Article 41189. https://doi.org/10.1038/srep41189

Journal Article Type Article
Acceptance Date Dec 12, 2016
Online Publication Date Jan 24, 2017
Publication Date Jan 24, 2017
Deposit Date May 19, 2017
Publicly Available Date Mar 29, 2024
Journal Scientific Reports
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 7
Article Number 41189
DOI https://doi.org/10.1038/srep41189

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http://creativecommons.org/licenses/by/4.0/

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/




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