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TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana

Espinosa-Ruiz, Ana; Martinez, Cristina; de Lucas, Miguel; Fabregas, Norma; Bosch, Nadja; Cano-Delgado, Ana I.; Prat, Salome

TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana Thumbnail


Authors

Ana Espinosa-Ruiz

Cristina Martinez

Norma Fabregas

Nadja Bosch

Ana I. Cano-Delgado

Salome Prat



Abstract

The transcription factor BRI1-EMS-SUPRESSOR 1 (BES1) is a master regulator of brassinosteroid (BR)-regulated gene expression. BES1 together with BRASSINAZOLE-RESISTANT 1 (BZR1) drive activated or repressed expression of several genes, and have a prominent role in negative regulation of BR synthesis. Here, we report that BES1 interaction with TOPLESS (TPL), via its ERF-associated amphiphilic repression (EAR) motif, is essential for BES1-mediated control of organ boundary formation in the shoot apical meristem and the regulation of quiescent center (QC) cell division in roots. We show that TPL binds via BES1 to the promoters of the CUC3 and BRAVO targets and suppresses their expression. Ectopic expression of TPL leads to similar organ boundary defects and alterations in QC cell division rate to the bes1-d mutation, while bes1-d defects are suppressed by the dominant interfering protein encoded by tpl-1, with these effects respectively correlating with changes in CUC3 and BRAVO expression. Together, our data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems, which relies on its interaction with BES1 and regulation of BES1 target gene expression.

Citation

Espinosa-Ruiz, A., Martinez, C., de Lucas, M., Fabregas, N., Bosch, N., Cano-Delgado, A. I., & Prat, S. (2017). TOPLESS mediates brassinosteroid control of shoot boundaries and root meristem development in Arabidopsis thaliana. Development, 144(9), 1619-1628. https://doi.org/10.1242/dev.143214

Journal Article Type Article
Acceptance Date Feb 27, 2017
Online Publication Date May 2, 2017
Publication Date May 2, 2017
Deposit Date Jun 8, 2017
Publicly Available Date Mar 29, 2024
Journal Development.
Print ISSN 0950-1991
Electronic ISSN 1477-9129
Publisher The Company of Biologists
Peer Reviewed Peer Reviewed
Volume 144
Issue 9
Pages 1619-1628
DOI https://doi.org/10.1242/dev.143214

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