Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Dubińska-Magiera, Magda; Chmielewska, Magdalena; Kozioł, Katarzyna; Machowska, Magdalena; Hutchison, Christopher J.; Goldberg, Martin W.; Rzepecki, Ryszard

doi:10.1007/s00709-015-0861-y

Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability

Dubińska-Magiera, Magda; Chmielewska, Magdalena; Kozioł, Katarzyna; Machowska, Magdalena; Hutchison, Christopher J.; Goldberg, Martin W.; Rzepecki, Ryszard

Authors

Magda Dubińska-Magiera

Magdalena Chmielewska

Katarzyna Kozioł

Magdalena Machowska

Christopher J. Hutchison

Professor Martin Goldberg m.w.goldberg@durham.ac.uk
Professor

Ryszard Rzepecki

Abstract

Xenopus LAP2β protein is the single isoform expressed in XTC cells. The protein localizes on heterochromatin clusters both at the nuclear envelope and inside a cell nucleus. The majority of XLAP2β fraction neither colocalizes with TPX2 protein during interphase nor can be immunoprecipitated with XLAP2β antibody. Knockdown of the XLAP2β protein expression in XTC cells by synthetic siRNA and plasmid encoded siRNA resulted in nuclear abnormalities including changes in shape of nuclei, abnormal chromatin structure, loss of nuclear envelope, mislocalization of integral membrane proteins of INM such as lamin B2, mislocalization of nucleoporins, and cell death. Based on timing of cell death, we suggest mechanism associated with nucleus reassembly or with entry into mitosis. This confirms that Xenopus LAP2 protein is essential for the maintenance of cell nucleus integrity and the process of its reassembly after mitosis.

Citation

Dubińska-Magiera, M., Chmielewska, M., Kozioł, K., Machowska, M., Hutchison, C. J., Goldberg, M. W., & Rzepecki, R. (2015). Xenopus LAP2β protein knockdown affects location of lamin B and nucleoporins and has effect on assembly of cell nucleus and cell viability. Protoplasma, 253(3), 943-956. https://doi.org/10.1007/s00709-015-0861-y

Journal Article Type	Article
Acceptance Date	Jul 15, 2015
Online Publication Date	Jul 25, 2015
Publication Date	Jul 25, 2015
Deposit Date	Jun 23, 2017
Publicly Available Date	Jun 23, 2017
Journal	Protoplasma
Print ISSN	0033-183X
Electronic ISSN	1615-6102
Publisher	Springer
Peer Reviewed	Peer Reviewed
Volume	253
Issue	3
Pages	943-956
DOI	https://doi.org/10.1007/s00709-015-0861-y

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