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In situ structural analysis of the Yersinia enterocolitica injectisome

Kudryashev, M.; Stenta, M.; Schmelz, S.; Amstutz, M.; Wiesand, U.; Castaño-Díez, D.; Degiacomi, M.T.; Münnich, S.; Bleck, C.K.E.; Kowal, J.; Diepold, A.; Heinz, D.W.; Dal Peraro, M.; Cornelis, G.R.; Stahlberg, H.

In situ structural analysis of the Yersinia enterocolitica injectisome Thumbnail


Authors

M. Kudryashev

M. Stenta

S. Schmelz

M. Amstutz

U. Wiesand

D. Castaño-Díez

S. Münnich

C.K.E. Bleck

J. Kowal

A. Diepold

D.W. Heinz

M. Dal Peraro

G.R. Cornelis

H. Stahlberg



Abstract

Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica and Shigella flexneri injectisomes in situ and the first structural analysis of the Yersinia injectisome. Unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had similar dimensions and were significantly longer than the isolated structures of related injectisomes. The crystal structure of the inner membrane injectisome component YscD appeared elongated compared to a homologous protein, and molecular dynamics simulations documented its elongation elasticity. The ring-shaped secretin YscC at the outer membrane was stretched by 30–40% in situ, compared to its isolated liposome-embedded conformation. We suggest that elasticity is critical for some two-membrane spanning protein complexes to cope with variations in the intermembrane distance.

Citation

Kudryashev, M., Stenta, M., Schmelz, S., Amstutz, M., Wiesand, U., Castaño-Díez, D., …Stahlberg, H. (2013). In situ structural analysis of the Yersinia enterocolitica injectisome. eLife, 2013(2), Article e00792. https://doi.org/10.7554/elife.00792

Journal Article Type Article
Acceptance Date Jun 27, 2013
Online Publication Date Jul 30, 2013
Publication Date Jul 30, 2013
Deposit Date Jul 26, 2017
Publicly Available Date Mar 29, 2024
Journal eLife
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 2013
Issue 2
Article Number e00792
DOI https://doi.org/10.7554/elife.00792

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© Copyright Kudryashev et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.





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