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Collision cross sections for structural proteomics

Marklund, E.G.; Degiacomi, M.T.; Robinson, C.V.; Baldwin, A.J.; Benesch, J.L.P.

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Authors

E.G. Marklund

C.V. Robinson

A.J. Baldwin

J.L.P. Benesch



Abstract

Ion mobility mass spectrometry (IM-MS) allows the structural interrogation of biomolecules by reporting their collision cross sections (CCSs). The major bottleneck for exploiting IM-MS in structural proteomics lies in the lack of speed at which structures and models can be related to experimental data. Here we present IMPACT (Ion Mobility Projection Approximation Calculation Tool), which overcomes these twin challenges, providing accurate CCSs up to 106 times faster than alternative methods. This allows us to assess the CCS space presented by the entire structural proteome, interrogate ensembles of protein conformers, and monitor molecular dynamics trajectories. Our data demonstrate that the CCS is a highly informative parameter and that IM-MS is of considerable practical value to structural biologists.

Citation

Marklund, E., Degiacomi, M., Robinson, C., Baldwin, A., & Benesch, J. (2015). Collision cross sections for structural proteomics. Structure, 23(4), 791-799. https://doi.org/10.1016/j.str.2015.02.010

Journal Article Type Article
Acceptance Date Feb 18, 2015
Online Publication Date Mar 19, 2015
Publication Date Apr 7, 2015
Deposit Date Jul 26, 2017
Publicly Available Date Aug 21, 2017
Journal Structure
Print ISSN 0969-2126
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 23
Issue 4
Pages 791-799
DOI https://doi.org/10.1016/j.str.2015.02.010

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