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The light-sheet microscopy revolution

Girkin, John M.; Carvalho, Mariana Torres

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Authors

Mariana Torres Carvalho



Contributors

E Carlaw d700tmt@durham.ac.uk
Other

Abstract

This paper reviews the rapid advances that have been made in one form of optical biological imaging in the last decade, namely that of light-sheet microscopy. Although the concept was originally presented over one hundred years ago, it was a methodology that lacked the technology to make it a viable tool for practical everyday imaging. However, since its renaissance, it has started to transform in vivo and, increasingly, intact organ imaging in multiple areas of biology. This review looks back at the beginning of the method and then focuses on the crucial role that modern optical technology, frequently developed for other fields, has played in advancing the instrumentation. We also look at the open-source route whereby, through the purchase of a few optical components, researchers have been able to develop their own bespoke instruments. We consider if this may be a route forward for the rapid development of other technological breakthroughs.

Citation

Girkin, J. M., & Carvalho, M. T. (2018). The light-sheet microscopy revolution. Journal of Optics, 20(5), Article 053002. https://doi.org/10.1088/2040-8986/aab58a

Journal Article Type Article
Acceptance Date Mar 9, 2018
Online Publication Date Apr 10, 2018
Publication Date May 1, 2018
Deposit Date Mar 12, 2018
Publicly Available Date Mar 28, 2024
Journal Journal of Optics
Print ISSN 2040-8978
Electronic ISSN 2040-8986
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 20
Issue 5
Article Number 053002
DOI https://doi.org/10.1088/2040-8986/aab58a

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Accepted Journal Article (4.5 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Original content from this work may be used under the terms
of the Creative Commons Attribution 3.0 licence. Any
further distribution of this work must maintain attribution to the author(s) and
the title of the work, journal citation and DOI.






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