Skip to main content

Research Repository

Advanced Search

Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM

Young, L.K.; Jarrin, M.; Saunter, C.D.; Quinlan, R.A.; Girkin, J.M.

Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM Thumbnail


Authors

L.K. Young

M. Jarrin

C.D. Saunter



Abstract

Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measuring the developing optical power of the eye lens, in a living animal, by exploiting the orthogonal imaging modality of a selective plane illumination microscope (SPIM). We have quantified the maturation of the lenticular refractive index at three different visible wavelengths using a combined imaging and ray tracing approach. We demonstrate that the method can be used with transgenic and vital dye labeling as well as with both fixed and living animals. Using a key eye lens morphogen and its inhibitor, we have measured their effects both on lens size and on refractive index. Our technique provides insights into the mechanisms involved in the development of this natural graded index micro-lens and its associated optical properties.

Citation

Young, L., Jarrin, M., Saunter, C., Quinlan, R., & Girkin, J. (2018). Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM. Biomedical Optics Express, 9(5), 2176-2188. https://doi.org/10.1364/boe.9.002176

Journal Article Type Article
Acceptance Date Mar 23, 2018
Online Publication Date Apr 10, 2018
Publication Date May 1, 2018
Deposit Date Mar 26, 2018
Publicly Available Date Mar 29, 2024
Journal Biomedical Optics Express
Publisher Optica
Peer Reviewed Peer Reviewed
Volume 9
Issue 5
Pages 2176-2188
DOI https://doi.org/10.1364/boe.9.002176

Files

Accepted Journal Article (1 Mb)
PDF

Copyright Statement
Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.







You might also like



Downloadable Citations