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Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via manipulating phenylpropanoid pathway and jasmonic acid synthesis

Hu, Q.; Min, L.; Yang, X.; Jin, S.; Zhang, L.; Li, Y.; Ma, Y.; Qi, X.; Li, D.; Liu, H.; Lindsey, K.; Zhu, L.; Zhang, X.

Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via manipulating phenylpropanoid pathway and jasmonic acid synthesis Thumbnail


Authors

Q. Hu

L. Min

X. Yang

S. Jin

L. Zhang

Y. Li

Y. Ma

X. Qi

D. Li

H. Liu

L. Zhu

X. Zhang



Abstract

Plants are constantly challenged by a multitude of pathogens and pests, which causes massive yield and quality losses annually. A promising approach to reduce such losses is to enhance the immune system of plants through genetic engineering. Previous work has shown that laccases (p-diphenol:dioxygen oxidoreductase, EC 1.10.3.2) function as lignin polymerization enzymes. Here we demonstrate that transgenic manipulation of the expression of the laccase gene GhLac1 in cotton (Gossypium hirsutum) can confer an enhanced defense response to both pathogens and pests. Overexpression of GhLac1 leads to increased lignification, associated with increased tolerance to the fungal pathogen Verticillium dahliae and to the insect pests cotton bollworm (Helicoverpa armigera) and cotton aphid (Aphis gosypii). Suppression of GhLac1 expression leads to a redirection of metabolic flux in the phenylpropanoid pathway, causing the accumulation of JA and secondary metabolites that confer resistance to V. dahliae and cotton bollworm; it also leads to increased susceptibility to cotton aphid. Plant laccases therefore provide a new molecular tool to engineer pest and pathogen resistance in crops.

Citation

Hu, Q., Min, L., Yang, X., Jin, S., Zhang, L., Li, Y., …Zhang, X. (2018). Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via manipulating phenylpropanoid pathway and jasmonic acid synthesis. Plant Physiology, 176(2), 1808-1823. https://doi.org/10.1104/pp.17.01628

Journal Article Type Article
Acceptance Date Dec 8, 2017
Online Publication Date Feb 6, 2018
Publication Date Feb 6, 2018
Deposit Date Mar 27, 2018
Publicly Available Date Mar 28, 2024
Journal Plant Physiology
Print ISSN 0032-0889
Electronic ISSN 1532-2548
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 176
Issue 2
Pages 1808-1823
DOI https://doi.org/10.1104/pp.17.01628

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Copyright Statement
© 2018 American Society of Plant Biologists.





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