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Enhancing multi-spot structured illumination microscopy with fluorescence difference

Ward, Edward N.; Torkelsen, Frida H.; Pal, Robert

Enhancing multi-spot structured illumination microscopy with fluorescence difference Thumbnail


Authors

Edward N. Ward

Frida H. Torkelsen



Abstract

Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.

Citation

Ward, E. N., Torkelsen, F. H., & Pal, R. (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science, 5(3), Article 171336. https://doi.org/10.1098/rsos.171336

Journal Article Type Article
Acceptance Date Feb 9, 2018
Online Publication Date Mar 14, 2018
Publication Date Mar 14, 2018
Deposit Date Mar 29, 2018
Publicly Available Date Mar 29, 2024
Journal Royal Society Open Science
Publisher The Royal Society
Peer Reviewed Peer Reviewed
Volume 5
Issue 3
Article Number 171336
DOI https://doi.org/10.1098/rsos.171336

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