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Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures

Liu, Jun; Higgins, Claire A.; Whitehouse, Jenna C.; Harris, Susan J.; Crawford, Heather; Christiano, Angela M.; Lako, Majlinda; Hole, Nicholas; Jahoda, Colin A.B.

Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures Thumbnail


Authors

Jun Liu

Claire A. Higgins

Jenna C. Whitehouse

Susan J. Harris

Heather Crawford

Angela M. Christiano

Majlinda Lako

Nicholas Hole



Abstract

In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within follicles in situ in a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.

Citation

Liu, J., Higgins, C. A., Whitehouse, J. C., Harris, S. J., Crawford, H., Christiano, A. M., …Jahoda, C. A. (2018). Hair Follicle Dermal Cells Support Expansion of Murine and Human Embryonic and Induced Pluripotent Stem Cells and Promote Haematopoiesis in Mouse Cultures. Stem Cells International, 2018, Article 8631432. https://doi.org/10.1155/2018/8631432

Journal Article Type Article
Acceptance Date Apr 26, 2018
Online Publication Date Aug 2, 2018
Publication Date Aug 2, 2018
Deposit Date Aug 30, 2018
Publicly Available Date Aug 30, 2018
Journal Stem Cells International
Print ISSN 1687-966X
Electronic ISSN 1687-9678
Publisher Hindawi
Peer Reviewed Peer Reviewed
Volume 2018
Article Number 8631432
DOI https://doi.org/10.1155/2018/8631432

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright © 2018 Jun Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.






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