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Measuring bilayer surface energy and curvature in asymmetric droplet interface bilayers.

Barlow, Nathan E. and Kusumaatmaja, Halim and Salehi-Reyhani, Ali and Brooks, Nick and Barter, Laura M. C. and Flemming, Anthony J. and Ces, Oscar (2018) 'Measuring bilayer surface energy and curvature in asymmetric droplet interface bilayers.', Journal of the Royal Society interface., 15 (148). p. 20180610.

Abstract

For the past decade, droplet interface bilayers (DIBs) have had an increased prevalence in biomolecular and biophysical literature. However, much of the underlying physics of these platforms is poorly characterized. To further our understanding of these structures, lipid membrane tension on DIB membranes is measured by analysing the equilibrium shape of asymmetric DIBs. To this end, the morphology of DIBs is explored for the first time using confocal laser scanning fluorescence microscopy. The experimental results confirm that, in accordance with theory, the bilayer interface of a volume-asymmetric DIB is curved towards the smaller droplet and a lipid-asymmetric DIB is curved towards the droplet with the higher monolayer surface tension. Moreover, the DIB shape can be exploited to measure complex bilayer surface energies. In this study, the bilayer surface energy of DIBs composed of lipid mixtures of phosphatidylgylcerol (PG) and phosphatidylcholine are shown to increase linearly with PG concentrations up to 25%. The assumption that DIB bilayer area can be geometrically approximated as a spherical cap base is also tested, and it is discovered that the bilayer curvature is negligible for most practical symmetric or asymmetric DIB systems with respect to bilayer area.

Item Type:Article
Full text:(VoR) Version of Record
First Live Deposit - 29 November 2018
Available under License - Creative Commons Attribution.
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Status:Peer-reviewed
Publisher Web site:https://doi.org/10.1098/rsif.2018.0610
Publisher statement:© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Record Created:29 Nov 2018 15:58
Last Modified:29 Nov 2018 16:03

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