Professor Robert Pal robert.pal@durham.ac.uk
Professor
In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy
Pal, Robert; Barker, Abigail C.J.; Hummel, Daniel; Pålsson, Lars-Olof
Authors
Abigail C.J. Barker
Daniel Hummel
Dr Lars- Palsson lars-olof.palsson@durham.ac.uk
Associate Professor
Abstract
In this work we demonstrate that time domain techniques can be used successfully to monitor realtively weak modulations of the fluorescence in sensing applications. The metal sensing complex Newport Green DCF™ can detect selected transition metals in vivo as well as in vitro. Incremental addition of Ni and/or Zn (in vitro) lead to a substantial reduction in the yield of the fast component in a bi-exponential fluorescence decay (τ1 = 150–250 ps) from 60% to 30–35%. This is rationalised as an inhibition of intra-molecular electron transfer in the NPG sensing complex due to metal complexation. In order to explore this effect in cellulo, NIH 3 T3 mouse skin fibroplast cells were pre-incubated with set levels of Ni and Zn, at a constant concentration of NPG. The fluorescence modulation in cellullo was subsequently studied employing both time-resolved fluorescence microscopy and confocal fluorescence microscopy. In correlation with the in vitro observations, similar effects were observed on the fluorescence decay in cellulo.
Citation
Pal, R., Barker, A. C., Hummel, D., & Pålsson, L. (2019). In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy. Journal of Fluorescence, 29(1), 255-263. https://doi.org/10.1007/s10895-018-2335-z
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 11, 2018 |
Online Publication Date | Dec 26, 2018 |
Publication Date | Jan 31, 2019 |
Deposit Date | Jan 4, 2019 |
Publicly Available Date | Jan 4, 2019 |
Journal | Journal of Fluorescence |
Print ISSN | 1053-0509 |
Electronic ISSN | 1573-4994 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
Volume | 29 |
Issue | 1 |
Pages | 255-263 |
DOI | https://doi.org/10.1007/s10895-018-2335-z |
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
Advance online version © The Author(s) 2018.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Published Journal Article
(1.7 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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