Holographic projection for multi-spot structured illumination microscopy

Ward, E.N.; Pal, R.

doi:10.1111/jmi.12787

Holographic projection for multi-spot structured illumination microscopy

Ward, E.N.; Pal, R.

Authors

E.N. Ward

Professor Robert Pal robert.pal@durham.ac.uk
Professor

Abstract

This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction‐limited imaging.

Citation

Ward, E., & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy, 274(2), 114-120. https://doi.org/10.1111/jmi.12787

Journal Article Type	Article
Acceptance Date	Feb 19, 2019
Online Publication Date	Feb 21, 2019
Publication Date	May 31, 2019
Deposit Date	Feb 22, 2019
Publicly Available Date	Feb 21, 2020
Journal	Journal of Microscopy
Print ISSN	0022-2720
Electronic ISSN	1365-2818
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	274
Issue	2
Pages	114-120
DOI	https://doi.org/10.1111/jmi.12787

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Copyright Statement
This is the accepted version of the following article: Ward, E. N. & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy 274(2): 114-120 which has been published in final form at https://doi.org/10.1111/jmi.12787. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.