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Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus L.) increases seed triacylglycerol content despite its low intrinsic flux control coefficient

Woodfield, Helen K.; Fenyk, Stepan; Wallington, Emma; Bates, Ruth E.; Brown, Alexander; Guschina, Irina A.; Marillia, Elizabeth-France; Taylor, David C.; Fell, David; Harwood, John L.; Fawcett, Tony

Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus L.) increases seed triacylglycerol content despite its low intrinsic flux control coefficient Thumbnail


Authors

Helen K. Woodfield

Stepan Fenyk

Emma Wallington

Ruth E. Bates

Alexander Brown

Irina A. Guschina

Elizabeth-France Marillia

David C. Taylor

David Fell

John L. Harwood



Abstract

Lysophosphatidate acyltransferase (LPAAT) catalyses the second step of the Kennedy pathway for triacylglycerol (TAG) synthesis. Here we express Trapaeolum majus LPAAT in Brassica napus (B. napus) cv 12075 to evaluate the effects on lipid synthesis and estimate the flux control coefficient for LPAAT. We estimate the flux control coefficient of LPAAT in a whole plant context by deriving a relationship between it and overall lipid accumulation, given that this is an exponential process. Increasing LPAAT activity resulted in greater TAG accumulation in seeds of between 25 and 29%; altered fatty acid distributions in seed lipids (particularly those of the Kennedy pathway); and a redistribution of label from 14C‐glycerol between phosphoglycerides. Greater LPAAT activity in seeds led to an increase in TAG content despite its low intrinsic flux control coefficient on account of the exponential nature of lipid accumulation that amplifies the effect of the small flux increment achieved by increasing its activity. We have also developed a novel application of metabolic control analysis likely to have broad application as it determines the in planta flux control that a single component has upon accumulation of storage products.

Citation

Woodfield, H. K., Fenyk, S., Wallington, E., Bates, R. E., Brown, A., Guschina, I. A., …Fawcett, T. (2019). Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus L.) increases seed triacylglycerol content despite its low intrinsic flux control coefficient. New Phytologist, 224(2), 700-711. https://doi.org/10.1111/nph.16100

Journal Article Type Article
Acceptance Date Jul 23, 2019
Online Publication Date Sep 14, 2019
Publication Date Oct 31, 2019
Deposit Date Aug 2, 2019
Publicly Available Date Mar 29, 2024
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 224
Issue 2
Pages 700-711
DOI https://doi.org/10.1111/nph.16100

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Advance online version © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.





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