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Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy

Suhling, Klaus; Hirvonen, Liisa M.; Becker, Wolfgang; Smietana, Stefan; Netz, Holger; Milnes, James; Conneely, Thomas; Marois, Alix Le; Jagutzki, Ottmar; Festy, Fred; Petrášek, Zdeněk; Beeby, Andrew

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Authors

Klaus Suhling

Liisa M. Hirvonen

Wolfgang Becker

Stefan Smietana

Holger Netz

James Milnes

Thomas Conneely

Alix Le Marois

Ottmar Jagutzki

Fred Festy

Zdeněk Petrášek



Abstract

Wide-field time-correlated single photon counting detection techniques, where the position and the arrival time of the photons are recorded simultaneously using a camera, have made some advances recently. The technology and instrumentation used for this approach is employed in areas such as nuclear science, mass spectroscopy and positron emission tomography, but here, we discuss some of the wide-field TCSPC methods, for applications in fluorescence microscopy. We describe work by us and others as presented in the Ulitima fast imaging and tracking conference at the Argonne National Laboratory in September 2018, from phosphorescence lifetime imaging (PLIM) microscopy on the microsecond time scale to fluorescence lifetime imaging (FLIM) on the nanosecond time scale, and highlight some applications of these techniques.

Citation

Suhling, K., Hirvonen, L. M., Becker, W., Smietana, S., Netz, H., Milnes, J., …Beeby, A. (2019). Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 942, Article 162365. https://doi.org/10.1016/j.nima.2019.162365

Journal Article Type Article
Acceptance Date Jul 10, 2019
Online Publication Date Jul 15, 2019
Publication Date Oct 21, 2019
Deposit Date Aug 29, 2019
Publicly Available Date Aug 29, 2019
Journal Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Print ISSN 0168-9002
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 942
Article Number 162365
DOI https://doi.org/10.1016/j.nima.2019.162365

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