Sanderson, J. M. and Ward, A. D. (2004) 'Analysis of liposomal membrane composition using Raman tweezers.', Chemical communications. (9). pp. 1120-1121.
We have developed a methodology for the analysis of liposomal membranes and their contents using near-IR Raman spectroscopy on liposomes held in an optical trap. We were able to detect a variety of membrane components including lipids, cholesterol, and small molecule solutes such as ethanol, DMSO and hexafluoroisopropanol. The methodology is able to distinguish between solutes that equilibrate across the liposomal membrane from those that partition selectively into the lipid bilayer.
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|Publisher Web site:||http://dx.doi.org/10.1039/b316757g|
|Record Created:||20 Apr 2007|
|Last Modified:||08 Apr 2009 16:30|
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