Analysis of Liposomal Membrane Composition Using Raman Tweezers

Sanderson, J.M.; Ward, A.D.

doi:10.1039/b316757g

Analysis of Liposomal Membrane Composition Using Raman Tweezers

Sanderson, J.M.; Ward, A.D.

Authors

Dr John Sanderson j.m.sanderson@durham.ac.uk
Associate Professor

A.D. Ward

Abstract

We have developed a methodology for the analysis of liposomal membranes and their contents using near-IR Raman spectroscopy on liposomes held in an optical trap. We were able to detect a variety of membrane components including lipids, cholesterol, and small molecule solutes such as ethanol, DMSO and hexafluoroisopropanol. The methodology is able to distinguish between solutes that equilibrate across the liposomal membrane from those that partition selectively into the lipid bilayer.

Citation

Sanderson, J., & Ward, A. (2004). Analysis of Liposomal Membrane Composition Using Raman Tweezers. Chemical Communications, 1120-1121. https://doi.org/10.1039/b316757g

Journal Article Type	Article
Acceptance Date	Mar 17, 2004
Online Publication Date	Apr 6, 2004
Publication Date	Apr 6, 2004
Deposit Date	Apr 20, 2007
Journal	Chemical Communications
Print ISSN	1359-7345
Electronic ISSN	1364-548X
Publisher	Royal Society of Chemistry
Peer Reviewed	Peer Reviewed
Issue	9
Pages	1120-1121
DOI	https://doi.org/10.1039/b316757g