CRISPR/Cas system: recent advances and prospects for genome editing

Manghwar, H.; Lindsey, K.; Zhang, X.; Jin, S.

doi:10.1016/j.tplants.2019.09.006

CRISPR/Cas system: recent advances and prospects for genome editing

Manghwar, H.; Lindsey, K.; Zhang, X.; Jin, S.

Authors

H. Manghwar

Professor Keith Lindsey keith.lindsey@durham.ac.uk
Professor

X. Zhang

S. Jin

Abstract

Genome editing (GE) has revolutionized biological research through the new ability to precisely edit the genomes of living organisms. In recent years, various GE tools have been explored for editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use, and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and Cas12a (Cpf1), are also considered.

Citation

Manghwar, H., Lindsey, K., Zhang, X., & Jin, S. (2019). CRISPR/Cas system: recent advances and prospects for genome editing. Trends in Plant Science, 24(12), 1102-1125. https://doi.org/10.1016/j.tplants.2019.09.006

Journal Article Type	Article
Publication Date	Dec 31, 2019
Deposit Date	Oct 1, 2019
Publicly Available Date	Nov 26, 2019
Journal	Trends in Plant Science
Print ISSN	1360-1385
Publisher	Cell Press
Peer Reviewed	Peer Reviewed
Volume	24
Issue	12
Pages	1102-1125
DOI	https://doi.org/10.1016/j.tplants.2019.09.006

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http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
© 2019 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/