Microsatellite marker development in the crop wild relative Linum bienne using genome skimming

Landoni, Beatrice; Viruel, Juan; Gomez, Rocio; Allaby, Robin G.; Brennan, Adrian C.; Pico, F. Xavier; Perez-Barrales, Rocio

doi:10.1002/aps3.11349

Microsatellite marker development in the crop wild relative Linum bienne using genome skimming

Landoni, Beatrice; Viruel, Juan; Gomez, Rocio; Allaby, Robin G.; Brennan, Adrian C.; Pico, F. Xavier; Perez-Barrales, Rocio

Authors

Beatrice Landoni

Juan Viruel

Rocio Gomez

Robin G. Allaby

Dr Adrian Brennan a.c.brennan@durham.ac.uk
Associate Professor

F. Xavier Pico

Rocio Perez-Barrales

Abstract

Premise: Nuclear microsatellite markers were developed for Linum bienne , the sister species of the crop L. usitatissimum , to provide molecular genetic tools for the investigation of L. bienne genetic diversity and structure. Methods and Results: Fifty microsatellite loci were identified in L. bienne by means of genome skimming, and 44 loci successfully amplified. Of these, 16 loci evenly spread across the L. usitatissimum reference nuclear genome were used for genotyping six L. bienne populations. Excluding one monomorphic locus, the number of alleles per locus ranged from two to 12. Four out of six populations harbored private alleles. The levels of expected and observed heterozygosity were 0.076 to 0.667 and 0.000 to 1.000, respectively. All 16 loci successfully cross‐amplified in L. usitatissimum . Conclusions: The 16 microsatellite loci developed here can be used for population genetic studies in L. bienne , and 28 additional loci that successfully amplified are available for further testing.

Citation

Landoni, B., Viruel, J., Gomez, R., Allaby, R. G., Brennan, A. C., Pico, F. X., & Perez-Barrales, R. (2020). Microsatellite marker development in the crop wild relative Linum bienne using genome skimming. Applications in Plant Sciences, 8(5), Article e11349. https://doi.org/10.1002/aps3.11349

Journal Article Type	Article
Acceptance Date	Jan 26, 2020
Online Publication Date	May 26, 2020
Publication Date	May 31, 2020
Deposit Date	May 26, 2020
Publicly Available Date	May 27, 2020
Journal	Applications in Plant Sciences
Electronic ISSN	2168-0450
Publisher	Botanical Society of America
Peer Reviewed	Peer Reviewed
Volume	8
Issue	5
Article Number	e11349
DOI	https://doi.org/10.1002/aps3.11349

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Copyright Statement
© 2020 Landoni et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.