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A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum

Liu, S.; Zhang, X.; Xiao, S.; Ma, J.; Shi, W.; Qin, T.; Xi, H.; Nie, X.; You, C.; Xu, Z.; Wang, T.; Wang, Y.; Zhang, Z.; Li, J.; Kong, J.; Aierxi, A.; Yu, Y.; Lindsey, K.; Klosterman, S.J.; Zhu, L.

A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum Thumbnail


Authors

S. Liu

X. Zhang

S. Xiao

J. Ma

W. Shi

T. Qin

H. Xi

X. Nie

C. You

Z. Xu

T. Wang

Y. Wang

Z. Zhang

J. Li

J. Kong

A. Aierxi

Y. Yu

S.J. Klosterman

L. Zhu



Abstract

Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW‐resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease‐resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR‐LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR‐based DNA marker (GhGLR4.8SNP(A/C)) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9‐mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8A results in a hypersensitive response. This first report of a GLR‐encoding gene that functions as an R gene provides a new insight into plant–pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7.

Citation

Liu, S., Zhang, X., Xiao, S., Ma, J., Shi, W., Qin, T., …Zhu, L. (2021). A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum. Advanced Science, 8(7), Article 2020723. https://doi.org/10.1002/advs.202002723

Journal Article Type Article
Online Publication Date Feb 19, 2021
Publication Date 2021
Deposit Date Feb 19, 2021
Publicly Available Date Mar 28, 2024
Journal Advanced Science
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 8
Issue 7
Article Number 2020723
DOI https://doi.org/10.1002/advs.202002723

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Early View © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.






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