Peptoids with Antibiofilm Activity against the Gram Negative Obligate Anaerobe, Fusobacterium nucleatum

Toole, Jamie; Bolt, Hannah L.; Marley, John J.; Patrick, Sheila; Cobb, Steven L.; Lundy, Fionnuala T.

doi:10.3390/molecules26164741

Peptoids with Antibiofilm Activity against the Gram Negative Obligate Anaerobe, Fusobacterium nucleatum

Toole, Jamie; Bolt, Hannah L.; Marley, John J.; Patrick, Sheila; Cobb, Steven L.; Lundy, Fionnuala T.

Authors

Jamie Toole

Hannah L. Bolt

John J. Marley

Sheila Patrick

Professor Steven Cobb s.l.cobb@durham.ac.uk
Professor

Fionnuala T. Lundy

Abstract

Peptoids (oligo N-substituted glycines) are peptide analogues, which can be designed to mimic host antimicrobial peptides, with the advantage that they are resistant to proteolytic degradation. Few studies on the antimicrobial efficacy of peptoids have focused on Gram negative anaerobic microbes associated with clinical infections, which are commonly recalcitrant to antibiotic treatment. We therefore studied the cytotoxicity and antibiofilm activity of a family of peptoids against the Gram negative obligate anaerobe Fusobacterium nucleatum, which is associated with infections in the oral cavity. Two peptoids, peptoid 4 (NaeNpheNphe)4 and peptoid 9 (NahNspeNspe)3 were shown to be efficacious against F. nucleatum biofilms at a concentration of 1 μM. At this concentration, peptoids 4 and 9 were not cytotoxic to human erythrocytes or primary human gingival fibroblast cells. Peptoids 4 and 9 therefore have merit as future therapeutics for the treatment of oral infections.

Citation

Toole, J., Bolt, H. L., Marley, J. J., Patrick, S., Cobb, S. L., & Lundy, F. T. (2021). Peptoids with Antibiofilm Activity against the Gram Negative Obligate Anaerobe, Fusobacterium nucleatum. Molecules, 26(16), Article 4741. https://doi.org/10.3390/molecules26164741

Journal Article Type	Article
Acceptance Date	Aug 3, 2021
Online Publication Date	Aug 5, 2021
Publication Date	2021
Deposit Date	Aug 24, 2021
Publicly Available Date	Aug 24, 2021
Journal	Molecules
Publisher	MDPI
Peer Reviewed	Peer Reviewed
Volume	26
Issue	16
Article Number	4741
DOI	https://doi.org/10.3390/molecules26164741

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