Cookies

We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.


Durham Research Online
You are in:

Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Erolb.

Dias-Gunasekara, S. and Gubbens, J. and Van Lith, M. and Dunne, C. and Williams, J. A. and Kataky, R. and Scoones, D. and Lapthorn, A. and Bulleid, N. J. and Benham, A. M. (2005) 'Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Erolb.', Journal of biological chemistry., 280 (38). pp. 33066-33075.

Abstract

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Understanding disulfide bond catalysis in mammals is important because of the involvement of protein misfolding in conditions such as diabetes, arthritis, cancer, and aging. Mammals express two related Ero proteins, Ero1 and Ero1. Ero1 is incompletely characterized but is of physiological interest because it is induced by the unfolded protein response. Here, we show that Ero1 can form homodimers and mixed heterodimers with Ero1, in addition to Ero-PDI dimers. Ero-Ero dimers require the Ero active site, occur in vivo, and can be modeled onto the Ero1p crystal structure. Our data indicate that the Ero1 protein is constitutively strongly expressed in the stomach and the pancreas, but in a cell-specific fashion. In the stomach, selective expression of Ero1 occurs in the enzyme-producing chief cells. In pancreatic islets, Ero1 expression is high, but is inversely correlated with PDI and PDIp levels, demonstrating that cell-specific differences exist in the regulation of oxidative protein folding in vivo.

Item Type:Article
Full text:Full text not available from this repository.
Publisher Web site:http://dx.doi.org/10.1074/jbc.M505023200
Record Created:14 May 2007
Last Modified:08 Apr 2009 16:31

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitterExport: EndNote, Zotero | BibTex
Usage statisticsLook up in GoogleScholar | Find in a UK Library