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The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution

Federici, L.; Du, D.; Walas, F.; Matsumura, H.; Fernandez-Recio, J.; McKeegan, K.S.; Borges-Walmsley, M.I.; Luisi, B.F.; Walmsley, A.R.

The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution Thumbnail


Authors

L. Federici

D. Du

F. Walas

H. Matsumura

J. Fernandez-Recio

K.S. McKeegan

M.I. Borges-Walmsley

B.F. Luisi

A.R. Walmsley



Contributors

M Borges-Walmsley vs000143@durham.ac.uk
Other

Abstract

Multidrug resistance in Gram-negative bacteria arises in part from the activities of tripartite drug efflux pumps. In the pathogen Vibrio cholerae, one such pump comprises the inner membrane proton antiporter VceB, the periplasmic adaptor VceA, and the outer membrane channel VceC. Here, we report the crystal structure of VceC at 1.8 Å resolution. The trimeric VceC is organized in the crystal lattice within laminar arrays that resemble membranes. A well resolved detergent molecule within this array interacts with the transmembrane -barrel domain in a fashion that may mimic proteinlipopolysaccharide contacts. Our analyses of the external surfaces of VceC and other channel proteins suggest that different classes of efflux pumps have distinct architectures. We discuss the implications of these findings for mechanisms of drug and protein export.

Citation

Federici, L., Du, D., Walas, F., Matsumura, H., Fernandez-Recio, J., McKeegan, K., …Walmsley, A. (2005). The crystal structure of the outer membrane protein VceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution. Journal of Biological Chemistry, 280(15), 15307-15314. https://doi.org/10.1074/jbc.m500401200

Journal Article Type Article
Publication Date Apr 15, 2005
Deposit Date May 14, 2007
Publicly Available Date Sep 21, 2016
Journal Journal of Biological Chemistry
Print ISSN 0021-9258
Electronic ISSN 1083-351X
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 280
Issue 15
Pages 15307-15314
DOI https://doi.org/10.1074/jbc.m500401200

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Copyright Statement
This research was originally published in Journal of Biological Chemistry. Federici, L., Du, D., Walas, F., Matsumura, H., Fernandez-Recio, J., McKeegan, K.S., Borges-Walmsley, M.I., Luisi, B.F. & Walmsley, A.R.. The Crystal Structure of the Outer Membrane Protein VceC from the Bacterial Pathogen Vibrio cholerae at 1.8 Å Resolution. Journal of Biological Chemistry. 2005. 280: 15307-15314. © the American Society for Biochemistry and Molecular Biology







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