Skip to main content

Research Repository

Advanced Search

A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain

Fordham-Skelton, AP; Chilley, P; Lumbreras, V; Reignoux, S; Fenton, TR; Dahm, CC; Pages, M; Gatehouse, JA

Authors

AP Fordham-Skelton

P Chilley

V Lumbreras

S Reignoux

TR Fenton

CC Dahm

M Pages

JA Gatehouse



Abstract

A novel protein phosphatase in Arabidopsis thaliana was identified by database searching. This protein, designated AtPTPKIS1, contains a protein tyrosine phosphatase (PTP) catalytic domain and a kinase interaction sequence (KIS) domain. It is predicted to interact with plant SNF1-related kinases (SnRKs), representing central regulators of metabolic and stress responses. AtPTPKIS1 has close homologues in other plant species, both dicots and monocots, but is not found in other kingdoms. The tomato homologue of AtPTPKIS1 was expressed as a recombinant protein and shown to hydrolyse a generic phosphatase substrate, and phosphotyrosine residues in synthetic peptides. The KIS domain of AtPTPKIS1 was shown to interact with the plant SnRK AKIN11 both in vivo in the yeast two-hybrid system, and in vitro in a GST-fusion 'pull down' assay. The genomes of Arabidopsis and other plants contain further predicted proteins related to AtPTPKIS1, which could also interact with SnRKs and act in novel regulatory and signalling pathways.

Citation

Fordham-Skelton, A., Chilley, P., Lumbreras, V., Reignoux, S., Fenton, T., Dahm, C., …Gatehouse, J. (2002). A novel higher plant protein tyrosine phosphatase interacts with SNF1-related protein kinases via a KIS (kinase interaction sequence) domain. The Plant Journal, 29(6), 705-715. https://doi.org/10.1046/j.1365-313x.2002.01250.x

Journal Article Type Article
Publication Date Mar 1, 2002
Deposit Date Feb 12, 2009
Journal Plant Journal
Print ISSN 0960-7412
Electronic ISSN 1365-313X
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 29
Issue 6
Pages 705-715
DOI https://doi.org/10.1046/j.1365-313x.2002.01250.x
Keywords Arabidopsis, SNRK, Domain fusion, Functional characterisation, Bioinformatics, Protein, Arabidopsis, Yeast, Identification, SNF1, Gene, Expression, Subunit, Complex, Glucose.