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The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase.

Schröder, M. and Clark, R. and Liu, C. Y. and Kaufman, R. J. (2004) 'The unfolded protein response represses differentiation through the RPD3-SIN3 histone deacetylase.', EMBO journal., 23 (11). pp. 2281-2292.


In Saccharomyces cerevisiae, splicing of HAC1 mRNA is initiated in response to the accumulation of unfolded proteins in the endoplasmic reticulum by the transmembrane kinase-endoribonuclease Ire1p. Spliced Hac1p (Hac1ip) is a negative regulator of differentiation responses to nitrogen starvation, pseudohyphal growth and meiosis. Here we show that the RPD3-SIN3 histone deacetylase complex (HDAC), its catalytic activity, recruitment of the HDAC to the promoters of early meiotic genes (EMGs) by Ume6p, and the Ume6p DNA binding site URS1 in the promoters of EMGs are required for nitrogen-mediated negative regulation of EMGs and meiosis by Hac1ip. Co-immunoprecipitation experiments demonstrated that Hac1ip can interact with the HDAC in vivo. Systematic analysis of double deletion strains revealed that HAC1 is a peripheral component of the HDAC. In summary, nitrogen-induced synthesis of Hac1ip and association of Hac1ip with the HDAC are physiological events in the regulation of EMGs by nutrients. These data also define for the first time a gene class that is under negative control by the UPR and provide the framework for a novel mechanism through which bZIP proteins repress transcription.

Item Type:Article
Keywords:Unfolded protein response, HAC1, RPD3, SIN3, Early meiotic genes.
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Record Created:15 May 2007
Last Modified:08 Apr 2009 16:31

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