Deeks, M. J. and Kaloriti, D. and Davies, B. and Malho, R. and Hussey, P. J. (2004) 'Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis.', Current biology., 14 (15). pp. 1410-1414.
The dynamic nature of the eukaryotic actin cytoskeleton is essential for the locomotion of animal cells and the morphogenesis of plant and fungal cells. The F-actin nucleating/branching activity of the Arp2/3 complex is a key function for all of these processes. The SCAR/WAVE family represents a group of Arp2/3 activators that are associated with lamellipodia formation [1 and 2]. A protein complex of PIR121, NAP1, ABI, and HSPC300 is required for SCAR regulation by cell signaling pathways , but the exact nature of this interaction is controversial and represents a continually evolving model . The mechanism originally proposed was of a SCAR trans repressing complex supported by evidence from in vitro experiments . This model was reinforced by genetic studies in the Drosophila central nervous system  and Dictyostelium , where the knockout of certain SCAR-complex components leads to excessive SCAR-mediated actin polymerization. Conflicting data have steadily accumulated from animal tissue culture experiments suggesting that the complex activates rather than represses in vivo SCAR activity [7, 8 and 9]. Recent biochemical evidence supports the SCAR-complex activator model . Here, we show that genetic observations in Arabidopsis are compatible with an activation model and provide one potential mechanism for the regulation of the newly identified Arabidopsis Arp2/3 complex.
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|Publisher Web site:||http://dx.doi.org/10.1016/j.cub.2004.06.065|
|Record Created:||16 May 2007|
|Last Modified:||20 Mar 2010 15:51|
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