OA Vaughan
Both emerin and lamin C depend on lamin A for localization at the nuclear envelope
Vaughan, OA; Alvarez-Reyes, M; Bridger, JM; Broers, JLV; Ramaekers, FCS; Wehnert, M; Morris, GE; Whitfield, WGF; Hutchison, CJ
Authors
M Alvarez-Reyes
JM Bridger
JLV Broers
FCS Ramaekers
M Wehnert
GE Morris
WGF Whitfield
CJ Hutchison
Abstract
Physical interactions between lamins and emerin were investigated by co-immunoprecipitation of in vitro translated proteins. Emerin interacted with in vitro translated lamins A, B1 and C in co-immunprecipitation reactions. Competition reactions revealed a clear preference for interactions between emerin and lamin C. Structural associations between lamins and emerin were investigated in four human cell lines displaying abnormal expression and/or localisation of lamins A and C. In each cell line absence of lamins A and C from the nuclear envelope (NE) was correlated with mis-localisation of endogenous and exogenous emerin to the ER. In two cell lines that did not express lamin A but did express lamin C, lamin C as well as emerin was mis-localised. When GFP-lamin A was expressed in SW13 cells (which normally express only very low levels of endogenous lamin A and mis-localise endogenous emerin and lamin C), all three proteins became associated with the NE. When GFP-lamin C was expressed in SW13 cells neither the endogenous nor the exogenous lamin C was localised to the NE and emerin remained in the ER. Finally, lamins A and C were selectively eliminated from the NE of HeLa cells using a dominant negative mutant of lamin B1. Elimination of these lamins from the lamina led to the accumulation of emerin as aggregates within the ER. Our data suggest that lamin A is essential for anchorage of emerin to the inner nuclear membrane and of lamin C to the lamina.
Citation
Vaughan, O., Alvarez-Reyes, M., Bridger, J., Broers, J., Ramaekers, F., Wehnert, M., …Hutchison, C. (2001). Both emerin and lamin C depend on lamin A for localization at the nuclear envelope. Journal of Cell Science, 114(14), 2577-2590
Journal Article Type | Article |
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Publication Date | Jul 1, 2001 |
Deposit Date | May 16, 2007 |
Journal | Journal of Cell Science |
Print ISSN | 0021-9533 |
Electronic ISSN | 1477-9137 |
Publisher | The Company of Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 114 |
Issue | 14 |
Pages | 2577-2590 |
Keywords | Lamins, Emerin, Nuclear envelope, Nuclear lamina, Emery-Dreifuss muscular dystrophy. |
Publisher URL | http://jcs.biologists.org/cgi/content/abstract/114/14/2577 |
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