Vaughan, O. A. and Alvarez-Reyes, M. and Bridger, J. M. and Broers, J. L. V. and Ramaekers, F. C. S. and Wehnert, M. and Morris, G. E. and Whitfield, W. G. F. and Hutchison, C. J. (2001) 'Both emerin and lamin C depend on lamin A for localization at the nuclear envelope.', Journal of cell science., 114 (14). pp. 2577-2590.
Physical interactions between lamins and emerin were investigated by co-immunoprecipitation of in vitro translated proteins. Emerin interacted with in vitro translated lamins A, B1 and C in co-immunprecipitation reactions. Competition reactions revealed a clear preference for interactions between emerin and lamin C. Structural associations between lamins and emerin were investigated in four human cell lines displaying abnormal expression and/or localisation of lamins A and C. In each cell line absence of lamins A and C from the nuclear envelope (NE) was correlated with mis-localisation of endogenous and exogenous emerin to the ER. In two cell lines that did not express lamin A but did express lamin C, lamin C as well as emerin was mis-localised. When GFP-lamin A was expressed in SW13 cells (which normally express only very low levels of endogenous lamin A and mis-localise endogenous emerin and lamin C), all three proteins became associated with the NE. When GFP-lamin C was expressed in SW13 cells neither the endogenous nor the exogenous lamin C was localised to the NE and emerin remained in the ER. Finally, lamins A and C were selectively eliminated from the NE of HeLa cells using a dominant negative mutant of lamin B1. Elimination of these lamins from the lamina led to the accumulation of emerin as aggregates within the ER. Our data suggest that lamin A is essential for anchorage of emerin to the inner nuclear membrane and of lamin C to the lamina.
|Keywords:||Lamins, Emerin, Nuclear envelope, Nuclear lamina, Emery-Dreifuss muscular dystrophy.|
|Full text:||Full text not available from this repository.|
|Publisher Web site:||http://jcs.biologists.org/cgi/content/abstract/114/14/2577|
|Record Created:||16 May 2007|
|Last Modified:||08 Apr 2009 16:31|
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